Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGGCTVKPQLLLLALVLHPWNPCLGADSEKPSSIPTDKLLVITVATKESDGFHRFMQSAKYFNYTVKVLGQGEEWRGGDGINSIGGGQKVRLMKEVMEHYADQDDLVVMFTECFDVIFAGGPEEVLKKFQKANHKVVFAADGILWPDKRLADKYPVVHIGKRYLNSGGFIGYAPYVNRIVQQWNLQDNDDDQLFYTKVYIDPLKREAINITLDHKCKIFQTLNGAVDEVVLKFENGKARAKNTFYETLPVAINGNGPTKILLNYFGNYVPNSWTQDNGCTLCEFDTVDLSAVDVHPNVSIGVFIEQPTPFLPRFLDILLTLDYPKEALKLFIHNKEVYHEKDIKVFFDKAKHEIKTIKIVGPEENLS-QAEARNMGMDFCRQDEKCDYYFSVDADVVLTNPRTLKILIEQNRK-----IIAPLVTRHGKLWSNFWGALSPDGYYARSEDYVDIVQGNRVGVWNVPYMANVYLIKGKTLRSEMNERNYFVRDKLDPDMALCRNAREMGVFM----YISNRHEFGRLLSTANYNTSHYNNDLWQIFENPVDWKEKYINRDYSKIFTENIVEQPCPDVFWFPIFSEKACDELVEEMEHYGKWSGGKHHDSRISGGYENVPTDDIHMK-QVDLENVWLHFIREFIAPVTLKVFAGYYTKGFALLNFVVKYSPERQRSLRPHHDASTF-TINIALNNVGEDFQGGGCKFLRYNCSIESPRKGWSFMHPGRLTHLHEGLPVKNGTRYIAVSFIDP 5TZJ Chain:A ((1-349)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDD--CSTDQTLQIVEKYRNKLN-LKVSQLETNSGGPGKPRNVALKQA----EGEFVLFVDSDDYI-NKETLKDAAAFIDEHHSDVLLIKMKGVNGRG--------VPQSMFKETAPEVTLLNSRIIYTLSPTKIYRTALLKDNDIY--FPEELKSAEDQLF-TMKAYLNANRISVLSDKAYYYATKRE-GEHMSSAYVSPEDFYEVMRLIAVEIL-------NADLEEAHKDQILAEFLNRHFSFS-------------------------------------RTNGFSLKVKLEEQPQWINALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGKLNIQFNEGEPYFEGID-----------------------------------------

General information: TITO was launched using: RESULT: Template: 5TZJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1804 -31037 -17.20 -92.10 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -17.20 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.262

(partial model without unconserved sides chains): PDB file : Tito_5TZJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TZJ-query.scw PDB file : Tito_Scwrl_5TZJ.pdb: