Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MEQLHFITKLLDIK---DPNIQILDIINKDTHKEIIAKLDYDAPSCPECGNQLKKYDFQKPSKIPYLETTGMPTRILLRKRRFKCYHCSKMMVAETSIVKKNHQIPRIINQKIAQKL-IEKISMTDIAHQLSISTSTVIRKLNDFHFKHDFSRLPEIMSWEVETVRGVTVSIGRWR------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ 5EHK Chain:A ((4-1114)) DSPFRAWDVFMVRTPVHLSLLRAACEDGLMEAVELASPSLAGLLARVARGDTGGLKDKRLRRAALALLRYDIRMRTRPTPFGLFAGVSGGRFDTSAKWLAGTGHRTRTRADMEWLLSAVHRLERDRVLLAGVTVQAHQTLTVRGDRIVLDCPSALGKSTVSARRSPVVAEILGAARRPVLAGRLAQSVAQRFELPADRVTGLLADMAAQELLITALRPPLDGDDPLQHVLDVVAAAEARAGSPAEAMSSESAALVAALREVDARCHAYDRTAVGQGRRELAELIQSTRRVHPHDTPLHVDLRIDLEVRLPEVVRTEIERAAEALWRLSPPRRGMRALRRYHEAFLERYGADRAVPLLELLDDTRGLGPPA--GYKWP----------------PSETPAGPQE--------EPRR------SAALARLVAKAARRGEREIVIDEETIAELAYDEAAPADLPNSLELGVHVVAPSLDELS-----AGTFRVVLAPGPGSHHAGATLGRFTGLLPDVDAESAARQAGRPLHIQDAVAADVAFIPRSGRAANLAHTPSYSGRRISVGLPDSGRAQEIPLDELGVGANLERLCLVHLPTGREVVPALPNMVSAFAQAPNPARLLFELGLEGQRLWEPWDWGALSEMPFLPGVRYGRTLLAAPLWRMDQLRGPADDSGPAADWDAALDRWRAEWNVPRRVLAVSMDQRLLLDLDDAWHRVLLRDELRRTPELIAQQVAGDEEGWLDFPGHLAEIVVPLERRDRHAARPPHIRATVSPTGAGGPWLYLRLRVPRRNQDDFLRDQVPVLVRAGIEHGADRWFFIRYSDTAGQHLRVRFRGEREKLWAGLLPEIGARLVEWQRQGLLAGHELGQYDPEYERYGGDALAEFTETAFQHDSAAAISLLRLTRRAGFRYTLDEVTAISAAALADAFGPPAPVVEPVPLVGGLQWAPDLFDGDPAAAWMSSTGGRRELPPDYRRDPARWQKLIDPTGGWPLLRADEDGCQVLAALESRDEAVRRFGTAYREAFRPTDSPSTQLRLVGSLLHMTCNRLIGGSAERERSVLGLARGAVQDNLNRRRH

General information: TITO was launched using: RESULT: Template: 5EHK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 328 21574 65.77 159.81 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : 65.77 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.161

(partial model without unconserved sides chains): PDB file : Tito_5EHK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EHK-query.scw PDB file : Tito_Scwrl_5EHK.pdb: