Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYHSLGNQFDTRTRTSRKIRRERSCSDMDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLEN--MYTETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAG-EGFVRIDTQNNMPTAAGLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGE------IYPVE--TDLK-LAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQ-SEKDYQDMLIYLKENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLRE-KGE-ACYFTMDAGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC 3D4J Chain:A ((8-332)) ------------------------------AAVTCTAPVNIAVIKYWGKRDEELVLPINSSLSVTLHQDQLKTTTTAVISKDF-TEDRIWLNGREED-VGQPRLQACLREIRCLARSCKVHVASVNNFPTAAGLASSAAGYACLAYTLARVYGV---ESDLSEVARRGSGSACRSLYGGFVEWQMGEQADGKDSIARQVAPESHWPELRVLILVVSAEKKLTGSTVGMRASVETSPLLRFRAESVVPARMAEMARCIRERDFPSFAQLTMKDSNQFHATCLDTFPPISYLNAISWRIIHLVHRFNAHHGDTKVAYTFDAGPNAVIFTLDDTVAEFVAAVWHGFPP--------------

General information: TITO was launched using: RESULT: Template: 3D4J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1617 1137 0.70 3.85 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 0.70 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.471

(partial model without unconserved sides chains): PDB file : Tito_3D4J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3D4J-query.scw PDB file : Tito_Scwrl_3D4J.pdb: