Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSYRIYSDMFDFA------V------DL---R---P-------NP-DYSLI--------QETIALMGDFLAV--RGQNLRPFSLAIYGKMEREGKKFGLGSSGSVVVLVVKALLALYNLS------------V---DQNLLFKLTSAVLLKRGDNGSMGDLACIAAEDLVLYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQQIKQNIN------QNFLTSSKETVVSLVEALE-------QGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQLKEASQDLQAVAKSSGAGGGDCGIALSFDAQST---KTLKNRWADLGIELLYQERIGHDDKS 2R3V Chain:C ((6-370)) -LLVSAPGKVILHGEHAVV-HGKVALAVSLNLRTFLRLQPHSNGKVDLSLPNIGIKRAWDVARLQSLDTSFL-VTTPTSEQVEKLKEVAGLPDDCAVTERLAVLAFLYLYLSICRKQRALPSLDIVVWSELP---PGAGLGSSAAYSVCLAAALLTVCEEIPNPLKDGDCVNRWTKEDLELINKWAFQGERMIHGNPSGVDNAVSTWGGALRYHQGK---I---------------------SSLKRSPALQILLTNTKVPRNTRALVAGVRNRLLKFPEIVAPLLTSIDAISLECERVLGEMGEAPAPEQYLVLEELIDMNQHHLNALG--VGHASLDQLCQVTRARGLHSKLTGAGGGGCGITLLKPGLEQPEVEATKQALTSCGFDCLET---------

General information: TITO was launched using: RESULT: Template: 2R3V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1324 -66225 -50.02 -225.26 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain C : 0.69 3D Compatibility (PKB) : -50.02 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.385

(partial model without unconserved sides chains): PDB file : Tito_2R3V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2R3V-query.scw PDB file : Tito_Scwrl_2R3V.pdb: