Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNHVASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVALGGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQPDGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVE-SILEHAETLIEHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGY-GKVMHGEAVAMGMVQISKIAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVPELGSATIHPV-SLEEMKDYLVK 5EKS Chain:A ((14-363)) ----VELGERRYPIFIGSQ-LDPK-QLLEPYIHGQQVMIVSNVTVAPLYLSHYQEALESLGKTVATCILPDGEKYKDIQHLNLIFDALLEAGFNRDCTVLALGGGVIGDMAGFASACFQRGVYFVQVPTTLLSQVDSSVGGKTGINHPLGKNMLGAFQQPQVVLADMAQLNTLPERELSAGLAEVIKYALLGDEDFLVWLEENMDGLVARDADLLAEAVYRSCAHKARIVANDEKE---RALLNLGHTFGHAIESYLGYGT-WLHGEAVATGMVMAADLSQRLGWISNEDVARTKKIIQRANLPISCPQIPLDDFLGYMAHDKKV---QLRLVLLKQLGQAVITKDFDVELMKQAIL-

General information: TITO was launched using: RESULT: Template: 5EKS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1904 -156470 -82.18 -458.86 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -82.18 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.545

(partial model without unconserved sides chains): PDB file : Tito_5EKS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EKS-query.scw PDB file : Tito_Scwrl_5EKS.pdb: