Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKRNNSQNLIMLKHDFASHIEDLKCATKPRSEALGRHLWTCTI---------DADFYWLKFHLPNVHAQSEQDFLHELQFYEDI-TYKKANWLLPFKIIEGRTISQQTQFQGKVLVLPDTECWFDDLDQ------------------KQNLKNINEKIYLALVKLAELHELGWIHGDIKKEHFRKFK-QELYLIDFEKTRLISSP-----DPITDATPRYMAPELFHGANKTVQSDLYALGIVLYEWLT-QTRLQANSYHEWAVLHC-QKLNVELPSS-FQIFLPLLSGLLQKQQQNRFSNVHEAINCLKALST 3TT0 Chain:A ((61-350)) -------------DPRWELPRDRLVLGKPLGEGAFGQ-VVLAEAIGLDKDKPNRVTKVAVKMLKSDATEKDLSDLISEMEMMKMIGKH--KNIINLLGACTQ------DGPLYVIVEYASKGNLREYLQARRPPGLEYSYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFGLARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPGVPVEELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAVPSQRPT-FKQLVEDLDRI--

General information: TITO was launched using: RESULT: Template: 3TT0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1147 -626 -0.55 -2.47 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -0.55 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.358

(partial model without unconserved sides chains): PDB file : Tito_3TT0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TT0-query.scw PDB file : Tito_Scwrl_3TT0.pdb: