Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREKMRFPTLVFDIETLTDLKAGAHLYHLDLPEADVEQALTKIRRQESGMDFQRLPLHEIVCISGLWIDESGFRLFSFSREHYSEAEILQKFLSIFDKRHP-TLVSWNGSQFDLPVILFRAMYH-GLSAPGLF-DQGELDS--QKRFNNYQNRY---HHRHIDLMDVMAMF---NGRNFQKLDDVACILGLPGKRGESGYHVPEYVRTEQWLKLTSYCEGDVLNTWFIYLRWLLLKGQMNVDEHNHWISSSIEYLQTMPQQADFLEVWQRTSKHTEFTSHYFNPLNF 1NOZ Chain:A ((188-340)) ---------------------------------------------------------------------------------FDNERDMLMEYINLWEQKRPAIFTGWNIEGFDVPYIMNRVKMILGERSMKRFSPIGRVKSKL-----S-KEIYSIDGVSILDYLDLYKKFAFTNLPS-FSLESVAQHETKKGKLPYD-GPINKLRET-NHQRYISYNIIDVESVQAIDKIRGFIDL--------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1NOZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 442 -1425 -3.22 -10.47 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -3.22 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.251

(partial model without unconserved sides chains): PDB file : Tito_1NOZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NOZ-query.scw PDB file : Tito_Scwrl_1NOZ.pdb: