Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MLNSEFQPNWLYPPSEVIKNFIQLNNIDTSAFDVSTKKIIDDLVNQNTKIDLYTSEVLKEAIGGTTQFWLNIQNQFTINSLRIQNSSVDNNFSEYRNLVNELKK-------LHWTPESKYDYLDQINLKSFF--GISEFSLLTKKYIVQ---HNLRVKFKNIGQYNSSE--------LNVATLIRKAELEAK---KQPILSPWN-----KLLFIEKLQEIKKLSKVKGFKNFRNELLSIC-----NQCGVAFIVLDT-----------LNKSPIRGI-SKFLDSNIGLIIVTTKYNKDDVFWQTFFHEAGHLVLHSKEMVFSDQGDLNKEASHNLEEAQADEFMLTHILHPYTIEEVTNMFNTRIMYKSKTESWRNICQIA-----RQVNISPSLLTGILKFQGIIPYSYFTNGHEKLYD------- 5LB8 Chain:D ((4-444)) PERRVRSTLKKVFGFDSFKTPLQESATMAVVKGNKDVFVCMPTGAGKSLCYQLPALLAKGITIVVSPLIALIQDQVDHLLTLKVRVSSLNSKLSAQERKELLADLEREKPQTKILYITPEMAASSSFQPTLNSLVSRHLLSYLVVDEAHCVSQWGHDFRPDYLRLGALRSRLGHAPCVALTATATPQVQEDVFAALHLKKPVAIFKTPCFRANLFYDVQFKELIS-DPYGNLKDFCLKACGIVYCRTREACEQLAIELSCRGVNAKAYHAGLKASERTLVQNDWMEEKVPVIVATISFDKANVRFVAHWNIAKSMAGYYQESGRA--GRDGKPSWCRLYYSRNDRDQVSFLIRK-EVAKLQEKRGNKASDKATIMAFDALVTFCEELGCRHAAIAKYFGDALPACAKGCDHCQNPTAVRRRLEALERSSS

General information: TITO was launched using: RESULT: Template: 5LB8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1766 39481 22.36 107.87 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain D : 0.65 3D Compatibility (PKB) : 22.36 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.109

(partial model without unconserved sides chains): PDB file : Tito_5LB8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LB8-query.scw PDB file : Tito_Scwrl_5LB8.pdb: