Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAVATDTQPLVGIIMGSQSDWATLEHTANMLKQLGVPFEAEVVSAHRTPDRLFEYAETARDRGIQVIIAGAGGAAHLPGMCAAKTDLPVLGVPVKSSILNGVDSLLSIVQMPAGIAVGTLAIGPAGATNAAIMAAQILGLTRPEIAKNVADFRAAQTDKVASNNIPGQV 5CVT Chain:B ((21-179)) -------SAPVVGIIMGSQSDFETMRHADALLTELEIPHETLIVSAHRTPDRLADYARTAAERGLNVIIAGAGGAAHLPGMCAA-TRLPVLGVPVESRALKGMDSLLSIVQMPGGVPVGTLAIGASGAKNAALLAASILALYNPALAARLETFRALQTASVPNSPI----

General information: TITO was launched using: RESULT: Template: 5CVT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 822 -133153 -161.99 -842.74 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.89 3D Compatibility (PKB) : -161.99 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.591

(partial model without unconserved sides chains): PDB file : Tito_5CVT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5CVT-query.scw PDB file : Tito_Scwrl_5CVT.pdb: