Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLYATQDEKKQAAAKAALK----HLPKGGILGVGTGSTVNFLIDLLPELQ--LEAAVASSQATADRLKKLGIEVVDMNHVVSLDAYVDGADEIDRHMHMIKGGGAALTREKIVASIAKKFVCIVDDSKWVDQLGRDFPLPVEVIPMARSAVARKLVSLGGDPVYREGVVTDNGNVILDVFNLNILNAIDLEKTINNIPGVVTNGIFALNPATIAIVATNDGIEERTAQ 4X84 Chain:B ((9-230)) ----MNQDQLKQAVAQAAVDHILPHLDSKSIVGVGTGSTANFFIDALARHKAEFDGAVASSEATAKRLKEHGIPVYELNTVSELEFYVDGADESNERLELIKGGGAALTREKIVAAVAKTFICIADASKLVPILG-QFPLPVEVIPMARSHVARQLVKLGGDPVYREGVLTDNGNIILDVHNLRIDSPVELEEKINAIVGVVTNGLFAARPADLLLLGTADGVKTLK--

General information: TITO was launched using: RESULT: Template: 4X84.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1271 8783 6.91 40.66 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.87 3D Compatibility (PKB) : 6.91 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.538

(partial model without unconserved sides chains): PDB file : Tito_4X84.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4X84-query.scw PDB file : Tito_Scwrl_4X84.pdb: