Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEKIKVRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNNLTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKTNLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQSTINSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTANKAQQIVNEHTIQ 2V66 Chain:B ((1-111)) -----------------------------------------------------------------------------------------------AEQRNRDLQADNQRLKYEVEALKEKLEHQYAQSYKQVSVLEDDLSQTRAIKEQLHKYVRELEQANDDLERAKRATIVSLEDFEQRLNQAIERNAFLESELDEKESLLVSVQ------------------------------

General information: TITO was launched using: RESULT: Template: 2V66.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 6 -110 -18.25 -0.99 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -18.25 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.743

(partial model without unconserved sides chains): PDB file : Tito_2V66.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2V66-query.scw PDB file : Tito_Scwrl_2V66.pdb: