Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTVSVDS-G-YKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMA-AYDRIGALGQQGHLAEVKLG--N--EAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAPSTFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGY-VFGDDG---TNPSDIGLNNAGAVEGISYATDWFK-NVW-PKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQTYKENNINYGVAKIPTLNNGQP-YQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEKFFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFDAASGS-KTPKESADEAVKTISEAIEQKY 2GH9 Chain:A ((2-379)) ------------------------------------------KITVWTHFGGPELEWLKEQARTFERTSGTKVEVVEVPFAEIKQKFILGAPQGQAADLVVTVPHDWVGEMAQAGVLEPVGKYVTQAYLADLQGVAVEAFTFGGRLMGLPAFAESVALIYNKKYVKEPPRTWEEFLALAQKLTT-----GAT-FGFLYNIGDPYFNFGFFKAFGAENVFAKDAKGNLDPTKLLIGGEVGEKALQFIKDLRFKYNLVPEGVD-----YGVADGAFKDGALAMILNGPWALGDYKKAKVDFGIAPFPVPPGAKNPWGPFLGVQGVVVNAYSKNKTQAVNFAKTLVTGRNLVAFNQAGGRIPVSKSAVKQ-L-EKDPVVAGFSKVFPLGAPMPNIPEMGKVWGPWGNAISLAIQRPDSNVKKIVEDMVAEIKKAIG---

General information: TITO was launched using: RESULT: Template: 2GH9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2040 48143 23.60 132.63 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 23.60 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.496

(partial model without unconserved sides chains): PDB file : Tito_2GH9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GH9-query.scw PDB file : Tito_Scwrl_2GH9.pdb: