Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRTRRLGQTLFSQPFLGEISMNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGTRDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEVVAITGSNGKTTTKDLTASVLSEKYKT-YKTQGNYNNNIGLPYTILHMPQDTEKIVLEMGMDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVPADEPLLTPLTATLVQTVTTFGIG-NGDVHAEVTAKGKAQTDFVVE-----GETFTIPLPGSYNVTNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTA---------GNGAAILSDVYNANPTAMGLVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLY-----GE------QMHALKEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK 4HV4 Chain:B ((121-480)) ----------------------------------------------------------------------------------------------------------------------YRHGIAVAGTHGKTTTTAMLSSIYAEAGLDPTFVNGG----LVKAAGTHARLGSSRYLIAEADESDAS-----FLHLQPMVAIVTNIEAD------DFENLKQTFINFLHNLPFYGRAVMCIDDPVVRELLPRVGRHITTYGFSDDADVQIASYRQEGPQGHFTLRRQDKPLIEVTLNAPGRHNALNAAAAVAVATEEGIEDEDILRALVGFQGTGRRFDFLGNFPLAPVNGKEGSAMLVDD-YGHHPTEVDATIKAARAGW-PDKRIVMLFQPHRYTR-TRDLYDDFANVLSQ--VDVLLMLDVYAAGEPPIPGADSRALCRTIRNRGK-LDPILVP-DSESAPEMLAQILNGEDLILVQGAGNIG--KIARKLAE--

General information: TITO was launched using: RESULT: Template: 4HV4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1753 509 0.29 1.56 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : 0.29 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.391

(partial model without unconserved sides chains): PDB file : Tito_4HV4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HV4-query.scw PDB file : Tito_Scwrl_4HV4.pdb: