Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAMTLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYLLKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLISEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK 4LE0 Chain:B ((5-131)) MISIFIAEDQQMLLGALGSLLNLEDDMEVVGKGTTGQDAVDFVKKRQPDVCIMDIEMPGKTGLEAAEE-LKDT-GCKIIILTTFARPGYFQRAIKAGVKGYLLKDSPSEELANAIRSVMNGKRIYAPEL---------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4LE0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 695 -77640 -111.71 -611.33 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : -111.71 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.581

(partial model without unconserved sides chains): PDB file : Tito_4LE0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LE0-query.scw PDB file : Tito_Scwrl_4LE0.pdb: