Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAQHVFIVGLGLIGASMALCIRQTNPELI--VVGWDSQSKTREQAVEQQIVDYVASDFET-GAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQVVTSAEQ-KNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKT-KKDVEKLQELFCGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDITRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKESRDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGILQISFKNQQDLLQAKRIIMSNTSYHCWIK 2G5C Chain:B ((2-280)) --QNVLIVGVGFMGGSFAKSLRRS--GFKGKIYGYDINPESISKAVDLGIIDEGTTSIAKVEDFSPDFVMLSSPVRTFREIAKKLSYIL-SEDATVTDQGSVKGKLVYDLENILGKRFVGGHPIAGTEKSGVEYSLDNLYEGKKVILTPTKKTDKKRLKLVKRVWEDVGGVVEYMSPELHDYVFGVVSHLPHAVAFALVDTLIHMSTPEVDLFKYPGGGFKDFTRIAKSDPIMWRDIFLENKENVMKAIEGFEKSLNHLKELIVREAEEELVEYLKEVKIKRME------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2G5C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1296 8416 6.49 30.72 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : 6.49 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.481

(partial model without unconserved sides chains): PDB file : Tito_2G5C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2G5C-query.scw PDB file : Tito_Scwrl_2G5C.pdb: