Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNRSLHGGHTTIHNNNAYHDAAKEERQLQEQQKPSMPGSHAAVERMTAVQVVDRLRWWRTHRVREQPIPVDHFVLRGSYANYSSFGTRPLAPSVTRLLQKARTVRAQYLRTQQQPQHEAPRQSRQVSSLLMAPTAGASVRSASNERLGSDAGETERTTGCISEEEGQYGERQQRQLREMNDDAKEKVNNKRRAAVIASWPRSPRPLNEVAIACLGEPAGQRDSATDNITRISLSASGSAGTSPPLVRAVSGSAPSVDTPSGALYVPSEQEARVAERLLMLFRPRAASAPLRSRRYRCTAGRLDATSSEFSGEPPLQGDWGHAEATGLHHERMTRVSASDEITDVRRAPGAAEAVIVLHHAPRLVLYEKGLHPKAALRAAGISSDALESLDDAAAESSAELFALRNLPLFAFERFFYQQQLRGLAETRRSHTPGMRSASADASAESAGVPESDTASAPTGAAAGAVAAADTAVDDDIIANGDDAFFFGSPLTATLACSLKTVVPLSSVAAAAAPGSRGRDGRDAAARQAVQHRGRGRDDGSNRRRGGRAALRAVSVPALPPVYKGGCPQMTLATSHHHLSSGARLQQQQQSSNRPSPILSAGLRSSKAPQNGAHLCLRVDDLQDVYVFNPVVDMIGKGAFSKVYAAVPILRGKEGLRRFASPLLDGQAAPCGSDELSGSGPREGGRRVSTVRRVSPVRRNSSSTALRVDPAAGAAVGDAAGPLTDDETAKRRPHAQSLAASLHSIPVVALKVIPRKARQKPKAAPDPLATTHSTAAAAAGSVASNAQQAAQNDQNSVRRELVEIEREVSILRSLHHTGCSQFFEAIRTPDAFVIAMRVFPGSMDAQRYLSRYGAPSEARAALLLFQLVSTVQYLHTNFGLIHRDIKLENILLSEADAGVPDARIREVLGPALHKSDTSAMMTEIEGGRKGCASATTAAETRTAAQRRYSMLSRNVARLLRVTLIDFGLARRTRASTLSPIVAASRGRGAGAQHGSLNATITSPTHLASFPPTSASLAASHPHASHNPNSSSYSGSLGSPASSSAGCAAGVVMLANGGNSSSNSYTPAKPAPPKPPLLCRPPSAAVGAGTARLGSSSAGISAMERSTPRAGMPSPMPSTANMFTRFLDLEEE-----MDEEENGGAAGTSNTTANTTTKVGDLGRGGRRDMAENEDDSGAFSTDVSASETDCESEGGSTA-EPKEGSAPNGEERLETGPQQEHVARKALISPSVLRAHPQTPPGSAIIVSPPLPPTPMLLSSTTAFTLDHFNAENSS-SSCNTSGPAAGGAGLRNRCASCIYTSHQQ------LSPAPPVV-------AAGVSSAPDDTEATLLLTPCGTEKYLPPEVLSWILEHGWARRSTTVGLARAMDLYSIGIVAYVLLSGCFPFNASSRATLLQQQQRVPRCNSARWAGVSSAAISFVQRLLEPNPRKRMTAKEALEHPFLHEARQLAEKLSLVPHGEGEEASHPSTWRDGSHMDNHHRHSIGGTGRHFEDDANVHARSHWATSTTATTNLPHSAPSPTHPDHTHSGAAIERASTQPSSSVSLAVYDSQRRHAGGPCELAAANEPSPLSSNSGADGGGELLCHHVESGTAPTATSISSGCRLSDAPRVCRSSDECEKDDVRASVARDVPGKVQPLLQACKESPYSTTPPVPVTPTLAKPCTAALARAASIELPALSSLVAHPAKPSPSSTLYFTATGTTASADGGRAERATLPEPESAAAGSSAPALAPGSKDAAAANRPVSLSPAATTSTARATEGGEDDLFESLYNNIMSSD 3DZO Chain:A ((13-382)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NLYFQGFRGTDPGDVVIEELFNRIPQANVRTTSEYAADSLVSTSLWNTGQPFRVESELGERPRTLVRGTVLGQEDPYAYLEATDQETGESFEVHVPYFTERAIKQMKEEVLRLRLLRGIKNQKQAKVHL------RFIFPFDLVKDPQVLSRFFLYPRMQSNLQTFGEVLLSHSSTHKSLVHHARLQLTLQVIRLLASLHHYGLVHTYLRPVDIVLDQRGGVFLTGFEHLVRDGASAV--SPIGR-GFAPPETTAERMLPFGQHHPTLMT--FAFDTWTLGLAIYWIWCADLPNTDDAALGGSEWIFRSCK-------NIPQPVRALLEGFLRYPKEDRLLPLQAMETPEYEQLRTELSAALPLYQ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3DZO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1663 61886 37.21 188.68 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 37.21 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.190

(partial model without unconserved sides chains): PDB file : Tito_3DZO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DZO-query.scw PDB file : Tito_Scwrl_3DZO.pdb: