Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAYNLSTSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSQDNGMMESFFGILKFMAMRKHLNHLINWNKLL 2X78 Chain:B ((122-236)) FDKFFIDYIGPLPPS-QGYLYVLVVVDGMTGFTWLYPTKAP-STSATVKSL-NVLTSI-AIPRVIHSDQGAAFTSSTFAEWAKERGIHLEFSTP-------KVERKNSDIKRLLT--------------

General information: TITO was launched using: RESULT: Template: 2X78.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 439 -48819 -111.20 -469.41 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : -111.20 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.328

(partial model without unconserved sides chains): PDB file : Tito_2X78.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2X78-query.scw PDB file : Tito_Scwrl_2X78.pdb: