Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIRFEEKVSTENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQALEGVFSIFDEQEFVGFIQKIRLEDSNLHIGRFFINPQKQEQGLGSQALRKFVSLAFENEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSILKLE 4PV6 Chain:O ((12-139)) ----TIREFSPKDIESVYRIAQTSLT---EYYTQALIL-DLHREWPESFMVYTVAGSVVGFIVGSKYSRTEARILLFAVDERFRRMGVGSALMDAFLSLC-REQNMLSVRLEVRTDNDEAIRFYKKYGFVITAMLPN-----------

General information: TITO was launched using: RESULT: Template: 4PV6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain O - contact count / total energy / energy per contact / energy per residue : 518 -83299 -160.81 -650.77 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain O : 0.70 3D Compatibility (PKB) : -160.81 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.424

(partial model without unconserved sides chains): PDB file : Tito_4PV6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PV6-query.scw PDB file : Tito_Scwrl_4PV6.pdb: