Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYKQTVQLEIISVNQTKTKIELVISQIEQQIKNRSLTPGTRLPSVRKLAKDLGFSVSTIVEAYERLIALGKIESRSGSGFYVVAPLAPLALNELGPKLDRSIDPLWISRQSLEAEPNAFKPGCGWLPNDWMPLESIRKALRSA-------------------TRS-------------EDDSLLGYSSPLGLSALRDLLARRAQS-KGIEANLNQVLLTDSGTQAIDLVCRFLLKPDDVVLIDDPCYFNFHALLKVHQVKVVGIPYTPNGPDLEAFKEAIEG-----YNPRLYITNSGIHNPTGATLSLSTAHQLLKLIDQSNLIVIEDDIFSDFEYTPA--------------------------PRLAALDNLSRVIFIGSFSKTLSASIRCGYIIAKPEWIDQIADLKIATSFSHNGLSAKILHTALTD---GSYRKHLDLLKVRLAQAMQETIAKLKSI-----RIEPWIEPKAGIFVWCRLPEGVD--------------AEKIAQFCLNRQVILAPGNAFSQAQS---------------AGQFIRFNITQSNHDYIY---KTLAEALLQESLEKQV 4JE5 Chain:B ((37-499)) ------------------------------------------------------------------------------------------------------------------DPNIIFLGGGLPLKDYFPWDNLSVDSPKPPFPQGIGAPIDEQNCIKYTVNKDYADKSANPSNDIPLSRALQYGFSAGQPELLNFIRDHTKIIHDLKYKDWDVLATAGNTNAWESTLRVFCNRGDVILVEAHSFSSSLASAEAQGVITFPVPIDADGIIPEKLAKVMENWTPGAPKPKLLYTIPTGQNPTGTSIADHRKEAIYKIAQKYDFLIVEDEPYYFLQMNPYIKDLKEREKAQSSPKQDHDEFLKSLANTFLSLDTEGRVIRMDSFSKVLAPGTRLGWITGSSKILKPYLSLHEMTIQAPAGFTQVLVNATLSRWGQKGYLDWLLGLRHEYTLKRDCAIDALYKYLPQSDAF-VINPPIAGMFFTVNIDASVHPEFKTKYNSDPYQLEQSLYHKVVERGVLVVPGSWFKSEGETEPPQPAESKEVSNPNIIFFRGTYAAVSPEKLTEGLKRLGDTLYEEF-----

General information: TITO was launched using: RESULT: Template: 4JE5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1694 11128 6.57 31.00 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : 6.57 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.507

(partial model without unconserved sides chains): PDB file : Tito_4JE5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JE5-query.scw PDB file : Tito_Scwrl_4JE5.pdb: