Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MECDVKPVRLAILGLGTVGGGALKLLQENAAEIKRRTGREIQITHVGTRRPRPDL----------EL---EG--IKQSADLLDIV----RQPDVDVVVEVMGGIHPAYEIIMEAIKHGKQVVTANKALLA---KHGNELFKAAEDNAVQIAYEAAVAGGIPIIKVIREGL-AANHIEWLAGIINGTGNFILTEMREK---GRAFDDVLKEAQELGYAEADPTFDVEGIDAAHKLTILASCAFGIPLQFDKVYTEGI-----SK----------ITA------QDVKYAEELGFRIKHLGIARRAEKGIELRVHPTLIPDEQLIANVNGVKNAVLVQANAVG-PTLYYGAGAGAGPTASAVVADVIDIVRDISYTEDGAGTIPQLAFEALTNMPILSREEMTTGYYIRLNAEDQTGVLADVTTILSRAGISIDAIMQQSRLKDLIPIVILTDPIVESKMDDALAQIQALPAIRGEIVRIRLESLDS 1EBU Chain:D ((1-357)) ---STKVVNVAVIGAGVVGSAFLDQLLAMKST------ITYNLVLLAEAERSLISKDFSPLNVGSDWKAALAASTTKTLPLDDLIAHLKTSPKPVILVDNTSS-AYIAGFYTKFVENGISIATPNKKAFSSDLATWKALFSNK-PTNGFVYHEATVGAGLPIISFLREIIQTGDEVEKIEGIFSGTLSYIFNEFSTSQANDVKFSDVVKVAKKLGYTEPDPRDDLNGLDVARKVTIVGRISGVEVESPTSFPVQSLIPKPLESVKSADEFLEKLSDYDKDLTQLKKEAATENKVLRFIGKVDVA--TKSVSVGIEKYDYSHPFASLKGSDNVISIKTKRYTNPVVIQGAGAGAAVTAAGVLGDVIKIAQR-------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1EBU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1477 -8647 -5.85 -27.98 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain D : 0.66 3D Compatibility (PKB) : -5.85 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.458

(partial model without unconserved sides chains): PDB file : Tito_1EBU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EBU-query.scw PDB file : Tito_Scwrl_1EBU.pdb: