Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGQYRVTALIPARGGSKRLPRKNVKLLVDKPLIAWSIEVAKASKYIDRVVVSTDDEEIKQVSEQYG-AEVPFLRPEYLSNDHASSFDVIKHAIDFLHLGQKNE-LIVLLQPTSPLRLVSELDTALEFFIAKNAKGIVSISETEHSP------MWSNT-LPENGCMSDFIRPEVQGKRS-----Q---DLPKFFRLNGSIYIYETLCLLEQ-----S---K------I--FF-NENVYGFETSLKTAIDIDTDLDFLIAETIMKNRAIE 3OAM Chain:A ((1-251)) ----MSFTVVIPARYQSTRLPGKPLADIGGKPMIQWVYEQAMQAG-ADRVIIATDDERVEQAVQAFGGVVC-MTSPNHQS-GTE----RLAEVVAKMA--IPADHIVVNVQGDEPLIPPAIIRQVADNLAACSAPMATLAVEIE-DEAEVFNPNAVKVITDKSGYALYFSRAT--IPWDRDNFAKADKAIVQPLLRHIGIYAYRAGFINTYLDWQPSQLEKIECLEQLRVLWHGEKIHVAVALEAPPAGVDTPEDLEVVRRIVAERA--

General information: TITO was launched using: RESULT: Template: 3OAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1043 -35698 -34.23 -164.50 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -34.23 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.477

(partial model without unconserved sides chains): PDB file : Tito_3OAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OAM-query.scw PDB file : Tito_Scwrl_3OAM.pdb: