Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQSVYHIPVKTISGETVDLDQYKGKVLLIVNTASKCGLT-PQYEGLEKLYQAKKDQGLEILGFPANNFKEQEPGSDEEIQQFCSLN-YDVHFPLFSKISVAGEDKHPLYQALTTAQPERIGEGPFRERLEGLGIPTNPAPEVLWNFEKFLVNKNGEVIARFAPNLTADDEQIVKAVEAELAK 3E0U Chain:A ((3-162)) --KSIYEFQVNAADGKPYDLSQHKGHPLLIYNVASRCGYTKGGYETATTLYNKYKGQGFTVLAFPCNQFAG-----------FAC-TRFKADFPIMAKIDVNGSKAHPLYEFMKATIPG--------LF---------GTKAIKWNFTSFLIDRHGVPVERFSPGASVED--IEKKLLPLLG-

General information: TITO was launched using: RESULT: Template: 3E0U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 714 -23598 -33.05 -160.53 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -33.05 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.583

(partial model without unconserved sides chains): PDB file : Tito_3E0U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E0U-query.scw PDB file : Tito_Scwrl_3E0U.pdb: