Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLGDFTMTDIVTRTELEEAKVDCKDLGDALNTKKVINPRYGEAFYSLPLAVQKVMETGGFEPFLTQAQLLSSTPTISPKAAKALDTGKIWYWGKGEGETEDSWHDTGLSELDQAISFTAEKMSFEYAEKLFKWEDLNKIPVMYLDALARLWLANLPKDVATHIGEFIDMQKSVYTQNGKNLNALYDSTGLPVAWITSDGKTVLPKIGVLTDYIENKVASVNRQYVDSVNQSAFLTDSLAVQTMGEFQQQQTVITAADVNAAAVFPHNVTKLRIPAITRIQKNKYLCFFEARLND-DDFGENSQGVCTLTVNESTFEITTSDIKALHAREVRPS--GGFYTFMNACATKLD-SGRVICLYVKRYG----------------TSEHYIYKRYSDDDGLTWSDYENIGTQLNMTFYNLLCPCS-QGLVKRYGSNNGRIIFPVWYSGKAYVASDFRAGYIYSDDGGTAWHDGAFAEIPYGNEVQCAEDLNGDLIFAIRLDQTVGGGTNDPTIQVKKLVKLLNGTLSTFIDIPA-PRLTNARVMSGLIQSKNAYD-RSTPKLQFICAND-LNRKNLKVWNSYDSGLNWTQ-YDVP-NTEVFAAYSCIEKLSYEKNLLLWEADNYSSLKTSIVSLRNLIGVA 4FJ6 Chain:D ((176-522)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DGSAAFRIPGLVTTNNGTLLGVYDIRYNSSVDLQEK--IDIGVSRSTDKG-QTWEPMRVAMTFKQTDGLPHGQNGVGDPSILVDEKTNTIWVVAAWTHGMGNERAWWNSMPGMTPDETAQLMLVKSEDDGKTWSEPINITSQVKDPSWYFLLQGPGRGITM----QDGTLVFPIQFIDAT---RVPNAGIMYSKDRGKTWHLHNLAR-TNTTEAQVAEVEPGVLMLNMRDNRGG----------SRAVATTKDLGKTWTEHPSSRSALQESVCMASLIKVNAKDNITGKDLLLFSNPNTTKGRNHITIKASLDGGLTWPTEHQVLLDEAEGWGYSCLSMIDKETVGIFYESSV-AHMTFQAVKLQDLIH--

General information: TITO was launched using: RESULT: Template: 4FJ6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2052 72054 35.11 224.47 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain D : 0.60 3D Compatibility (PKB) : 35.11 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.355

(partial model without unconserved sides chains): PDB file : Tito_4FJ6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FJ6-query.scw PDB file : Tito_Scwrl_4FJ6.pdb: