Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKVKTVYRCEQCGADHLKWAGQCSECGEWNCLNEVKLEPTTAHRARPKVGGYAGQVANITTLNKVSVSHETRLPTGISEFDRVLGGGLVTGSVVLIGGDPGIGKSTILLQTATHMASAK-SSALYITGEESLSQVALRAQRLDLPTDQLK----------------------VMAETCVERICEVLEQERPVVAILDSIQTLYTETLQSAPGGVSQIRESAALLTRYAKNSG-TALFIVGHVTKEGALAGPRVLEHMVDCVLYFEGQSD--SRYRMIRAVKNR-FGAVNELGVFGMTDKGLREVANPSAIF------------LSRYDEAIPG------SIVMISREGTRPLLVEVQALVDDAQGQPRRVALGLEQNRLNMLLAV---------MHRHGGVQTTGQDVYVN---------------------IVGGLKITETGSDLAVLLACASSLRTKALPQQLAVFGEVGLSGEIRPVPNGQERLKEAAKHGFKYVILPRGNAPQKAIPGVQVIAVA-----RLHEALTEAMQLSDELT------------- 1TF7 Chain:A ((14-497)) ------------------------------------------------------------------EHQAIAKMRTMIEGFDDISHGGLPIGRSTLVSGTSGTGKTLFSIQFLYNGIIEFDEPGVFVTFEETPQDIIKNARSFGWDLAKLVDEGKLFILDASPDPEGQEVVGGFDLSALIERINYAIQKYRARRVSIDSVTSV-----FQQYDASSVVRRELFRLVARLKQIGATTVMTTERIEEYGPIARYGVEEFVSDNVVILRNVLEGERRRRTLEILKLRGTSHMKGEYPFTITDHGINIFPLGAMRLTQRSSNVRVSSGVVRLDEMCGGGFFKDSIILATGATGTGKTLLVSRFVENACANKERAILFAYEESRAQLLRNAYSWGMDFEEMERQNLLKIVCAYPESAGLEDHLQIIKSEINDFKPARIAIDSLSALARGVSNNAFRQFVIGVTGYAKQEEITGLFTNTSDQFMGAHSITDSHISTITDTIILLQYVEIRGEMSRAINVFKMRGSWHDKAIREFMISDKGPDIKDSFRNFERIISGSPTRI

General information: TITO was launched using: RESULT: Template: 1TF7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1998 -46127 -23.09 -117.97 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -23.09 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.398

(partial model without unconserved sides chains): PDB file : Tito_1TF7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TF7-query.scw PDB file : Tito_Scwrl_1TF7.pdb: