Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWNPNSIIFCFYFIFSILALWGIIEAWVHQSRTETIHPFKAFVHLLAFYLSYLLIPLWFFNLYAGWIGYYSIHETIFIFLLSGILIYARFIEPHMVRVDVHQYRLNPDRHFVKPVKVALIADLHIGLFSGH-------ERQLKIIVKKLNEQQPDLVVVAGDWTYEPED---KLVQELSVLKD------------------IKAPVYSVPGNHDEQYPGPPIQQLLKDALYYNDVM-DIEG---------KIVEF----DEFRLIGIGDLWAG-------KTDMRSMPDL--PQDKPWLILSHNPDTVDM-------VPKLPNRPLMLSGHTHGGQVELPLVTNYIMKKVSILGHKRGFYSHEHADVFVTVGTGMVGIPLRFRVPPTIDIIELA 3T1I Chain:A ((27-273)) ------------------------------------------------------------------------------------------------------------LDDENTFKILVATDIHLGFMEKDAVRGNDTFVTLDEILRLAQENEVDFILLGGDLFHENKPSRKTLHTCLELLRKYCMGDRPVQFEILSDQLNISIPVFSIHGNHDDPTGADALC--ALDILSCAGFVNHFGRSMSVEKIDISPVLLQKGSTKIALYGLGSIPDERLYRMFVNKKVTMLRPKEDENSWFNLFVIHQNRSKHGSTNFIPEQFLDDFIDLVIWGHEHECKIAP-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3T1I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 759 9372 12.35 54.81 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : 12.35 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_3T1I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3T1I-query.scw PDB file : Tito_Scwrl_3T1I.pdb: