Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence KCVIKRVFSDGSSVKIPAQPA------QSTTISKQAVWVYGNCQPFP------VVLNDGSKLSGEIETPAVTPVES 1TYG Chain:B ((22-86)) --MLQ-L--NGKDVKWKKDTGTIQDLLASYQLENKIVIVERNKEIIGKERYHEVELCDRDVI--EI----VHFVGG

General information: TITO was launched using: RESULT: Template: 1TYG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 142 -3358 -23.65 -63.36 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : -23.65 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.648

(partial model without unconserved sides chains): PDB file : Tito_1TYG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TYG-query.scw PDB file : Tito_Scwrl_1TYG.pdb: