Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCAAQMPPLAHIFRGT-FVHSTWTCPMEVLRDHLLGVSDSGKIVFLEEASQQEKLAKEWCFKPCEIRELSHHEFFMPGLVDTHIHASQYSFAGSSI--DLPLLEWLTKYTF-----PAEHRFQNIDFAEEVYTRVVRRTLKNGTTTACYFATIHT-----DSSLLLADITDKFGQRAFVGKVCMDLNDTFPEYK-----ETTEESIKETERFVSEMLQ-KNYSRVKPIVTPRFSLSCSETLMGELGNIAKTRDLHIQSHISENRDEVEAVKNLYPSYKNYTSVYDKNNLLTNKTVMAHGCYLSAEELNVFHERGASIAHCPNSNLSLSSGFLNVLEVLKHEVKIGLGTDVAGGY-SYSMLDAIRRAVMVSNILLINKVNEKSLTLKEVFRLATLGGSQALGLDGEIGNFEVGKEFDAILINPKASDSPIDLFYGDFFGDISEAVIQKFLYLGDDRNIEEVYVGGKQVVPFSSSV 5HME Chain:A ((2-438)) --------RMILIRGLTRVITFDD-QERELEDADILID-GPKIVAVGKNLSDR---------SVSRTIDGRGMIALPGLINSHQHLYEGAMRAIPQLERVTMASWLEGVLTRSAGWWRDGKFG-PDVIREVARAVLLESLLGGITTVADQHPFFPGATADSYIDATIEAATDLGIRFHAVRSSMTLGKSEGGFCDDLFVEPVDRVVQHCLGLIDQYHEPEPFGMVRIALGPCGVTHDKPELFEAFAQMAADYDVRLHTHFYEPLDAG-MSDHLY-G-MTPWRFLEKHGWASDRVWLAHAVVPPREEIPEFADAGVAIAHLIAPDLRLGWGLAPIREYLDAGITVGFGTTGSASNDGGNLLGDLRLAALAHRPADPN-EPEKWLSARELLRMATRGSAECLGRP-DLGVLEEGRAADIACWRLDGVD-RV----------GVHDPAIGLIMTGLSDRASLVVVNGQVLVENERP-

General information: TITO was launched using: RESULT: Template: 5HME.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2507 -42812 -17.08 -102.67 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -17.08 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.457

(partial model without unconserved sides chains): PDB file : Tito_5HME.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HME-query.scw PDB file : Tito_Scwrl_5HME.pdb: