Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFP----NTVFAGFYLFDGKE---LVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYISCDSLAKSEIVVPMM-KNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTMFEEKS 1F5M Chain:A ((4-179)) STGFHHADHVNYSSNLNKEEILEQLLLSYEGLSDGQVNWVCNLSNASSLIWHAYKSLAVDINWAGFYVTQASEENTLILGPFQGKVACQMIQFGKGVCGTAASTKETQIVPDVNKYPGHIACDGETKSEIVVPIISNDGKTLGVIDIDCLDYEGFDHVDKEFLEKLAKLINKSCVF---------

General information: TITO was launched using: RESULT: Template: 1F5M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 715 -3444 -4.82 -22.08 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -4.82 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.596

(partial model without unconserved sides chains): PDB file : Tito_1F5M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1F5M-query.scw PDB file : Tito_Scwrl_1F5M.pdb: