Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLIVSVMPRSLEEAQAL-DATRYLDADIIEWRADYLPKEAILQVAPAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKE-VAQLYQPDYIDFEYYSYKDVFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNYTRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKIREILDEA 3L9C Chain:A ((1-224)) MKIVVPVMPQNIEEANQLDLTRID-STDIIEWRADYLVKDDILTVAPAIFEKFSGHEVIFTLRTEKEGGNISLSNEDYLAIIRDIAA-LYQPDYIDFEYFSYRDVLEEMYDFSNLILSYHNFEETPENLMEVFSELTALAPRVVKIAVMPKNEQDVLDLMNYTRGFKTLNPNQEYVTMSMSKLGRISRLAADLIGSSWTFASL-------QISLADMRKIKEVLDA-

General information: TITO was launched using: RESULT: Template: 3L9C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1097 -35439 -32.30 -164.83 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.92 3D Compatibility (PKB) : -32.30 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.571

(partial model without unconserved sides chains): PDB file : Tito_3L9C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3L9C-query.scw PDB file : Tito_Scwrl_3L9C.pdb: