Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGDYIDPELLTQFTEETGIQVQYEAFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDLLVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDE-APEHWDDLWKLEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDPQQLEETVDKLYKLTPNIKAIVADEMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDSAYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYEKFDHKWTGKYSDLFLQFKMYRK 4GL0 Chain:A ((10-332)) ---------------------------------SNTLTIYNWGDYIDPSLITKFEKETGIKVIYQTFDSNEAMMTKIEQGGTTFDIAVPSDYAISKMKEENLLIPLDHSKLPNEKYLDPRFMDLSFDDDNKYSMPYFWGTLGIIYNKEMFPDKNFDTWNALFDPELKNQILLIDGAREVMGLGLNSLGYSLNDTNKAHLQAARDKLETMTPNVKAIVGDEIKLLMADN-AGVAVTFSGEAAEMLSENEDLEYVIPKDGSNLWFDNMVIPKTAKNVDGAHKFINFMLKPENAAINAEYVGYATPNAKAVELLPKEISSDERFYPDMDELNNLEVYDNLGKRMLSYYNELFLEFKMYR-

General information: TITO was launched using: RESULT: Template: 4GL0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1801 -50049 -27.79 -155.91 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -27.79 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.492

(partial model without unconserved sides chains): PDB file : Tito_4GL0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GL0-query.scw PDB file : Tito_Scwrl_4GL0.pdb: