Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKAITDATFEQET--KDGLVLVDFWATWCGPCRMQGPILDKLSEELSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTVEQIKAIIAELS 2FCH Chain:B ((7-103)) ----LTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEY-QGKLTVAKLNIDQNPGTAPKYGIRSIPTLLLFKNGEVAATKVGALSKGQLKEFL----

General information: TITO was launched using: RESULT: Template: 2FCH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 378 834 2.21 8.77 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : 2.21 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.645

(partial model without unconserved sides chains): PDB file : Tito_2FCH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FCH-query.scw PDB file : Tito_Scwrl_2FCH.pdb: