Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVTTYIGLGSNLGDSRQILSEAITKLKSLGEV------KVSKLYQSPPMGPQDQPNYLNAVAQLKTELLPLELLDQLQRFEQEAGRVR-LRHWGERTLDLDLLIYGNEKIQNERLTVPHVGILQRDFVVIPLLDLDADLHINDQPLKNLELIQQPTLTVLADESWA 3HSD Chain:B ((1-139)) --TVAYIAIGSNLASP---LEQVNAALKALGDIPESHILTVSSFYRTPPLGPQDQPDALNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLFGNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPD-----------------------

General information: TITO was launched using: RESULT: Template: 3HSD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 628 10944 17.43 82.91 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : 17.43 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_3HSD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HSD-query.scw PDB file : Tito_Scwrl_3HSD.pdb: