Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQTLRASKCGLSTAQLPSSILDVIDSLTKAG----YEAYIVGGGVRDLMLGLNPKDFDAVTNATPSQIKEVFGRR---CRIIGRRFELAHVYSG-RELIEVATFRAPPKKAVTSASGMILRDNNWGTIEQDFARRDFSINTLYYQPRK---GIVLDFCKAIDDVKSKTLRLLGDPVQRFEEDPVRMLRTLRFAAKLNFKIDPAILDIFDVEMT--QLLRDVSPHRLYDESQKLFTMGHLARVLPMLIAFGVWKQLFADIRPDLT--PFIQRAAKNTDQRIQVGKTINPAFFYAVLLWQPFLERCDFYLSKGVVPAEARAQAGLDVLKRQATRTVIPRFAETFIREVWEMQTRLLNPKPQQIEALASHARFRAGFDFLLLREKSGDDTTQGMGSWWEAYQEMSNDEKEAAISQYNRQKAKSRRKVAAEPVESNKVDTEIEPLVDVPEPRSRRGKKERARQEQSIDRFIEKSSAAQTNVMSDHPILKRKRVQRDLSQVIFGPTQ 5HC9 Chain:A ((12-326)) ------------VERVDPKILNLFRLLGKFGDEVNMPVYVVGGFVRDLLLGIKNLDIDIVVEGNALEFAEYAKRFLPGKLVKHDKFMTASLFLKGGLRIDIATARLEYYE----SPAKL-PDVEMSTIKKDLYRRDFTINAMAIKLNPKDFGLLIDFFGGYRDLKEGVIRVLHT--LSFVDDPTRILRAIRFEQRFDFRIEETTERLL-KQAVEEGYLERTTGPRLRQELEKILEEKNPLKSIRRMAQFDVIKHLFPKTYYTPSMDEKMENLFRNIPWV---EENFGEVDRFYAVLHVFLEFY------------------DDESWKEVRDRYSLRRNLINEIRHVEKSAPALLEM--------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5HC9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1346 17563 13.05 58.54 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 13.05 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_5HC9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HC9-query.scw PDB file : Tito_Scwrl_5HC9.pdb: