Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSMIDILQLEGVQPIDYRNEDGCIMITVQPS-GEHLETC---PECGGRLYKHGQRINHFADTPLQMQPVKIEVIRTRYRCSECKSMITSQLSFLDEKRRATHRLIQQVRKRCLDRTFTQLAEDTGVVVNTIKNITLDFVEELERDIKFETPTIMGIDELKLMGTYRCVITNLAMNSLYDMLPERTQDTLIPYFAKLPDAEKVEWICSDMWRPFKKSFRLHLPNAKLIIDKFHVVRMA-SEALDTERKALQSSLDRDARLNMKKHLRWILLRRPNSLTEDQQRILGNLEKWHPEFKEAYDLKEQFYNIY-----EATTKDDAIQR---FHEWESSIPKYLKSFRDVAKTVNNNFEDIFTYWD-APIRITIAYT---EGHNGITRVANRMGRGYTFEVLRAKMLYNKVARSITTLKTPSSSLKSTKGYEGLTAFPTKQEKTKFEYGAYI---PTLVELYGGDEDLDEELT- 4N0N Chain:A ((22-422)) ---SAVCTVCGAAPVAKSACGGWFCGNCVPYHAGHCHTTSLFANCGHDIMYRSTYCTMCEGSPKQMVP------------KVPHPILDHLLCHIDYGSKEELTLVVA-DGRTTSPPGRYKVGHKVVAVV--ADVGGNIVFGCGPGSHIAVP---------LQDTLKGVVVNKALKNAAAS----------EYVEGPPGSGKTFHLVKDVLAV-VGSATLVVPTHASMLDCINKLKQAGADPYFVVPKYTVLDFPRPGSGN-------ITVRLPQVGTSEGETFVDEVAYFSP-VDLARILTQGRVKGYGDLNQLGCVGPASVPRNLWLRHFVSLEP--LRVCHRFGAAVCDLIKGIYPYYEPAPHTTKVVFVPNPDFEKGVVITAYHKDRG----------LGHRTIDSIQGCTFPVVTLR----------LPTPQSLTRPRAVVAVTRASQELYIYDPFDQLSGLLKF

General information: TITO was launched using: RESULT: Template: 4N0N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1964 2939 1.50 7.73 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 1.50 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.129

(partial model without unconserved sides chains): PDB file : Tito_4N0N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4N0N-query.scw PDB file : Tito_Scwrl_4N0N.pdb: