Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRFAINGYGEAQDVFEEIDAHPR-EVGSGHVRVEIKAFSVNPYDVALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHA----------------------------VGGTYGEEVVLPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLIQLLHEKGIRILTSASS-KNEEKVKKLGASAFAAYDKTNPGLQF----A-DQADLVIDATKGSIKGETGIQIMKPGGRYVALNDLPDLD--LRQKK---EGFYESFVPRKEYLDAEAFAGIIKAYQKGAFHVFISMNLSASLKHVIQAHQLVEG-HPPAGKIILSFEK 5K1S Chain:C ((18-360)) MKAVVLRSFGEA-GNLKMETM-PMPRPGRGEVLLRVHACGVCYHDVINRRGNLP---RTSVPAILGHEAAGEVIEVGPDTPGWKTGDRAATLQRMSCGDCALCRSGRNSLCKTDNRFFGEELPGGYAQFMVAPVGGLGRVPASLPWNEAATVCCTTGTAVHTVRTRGKVRAGETVLITGASGGVGLSSVQLARLDGARVIAVTSSEAKVQALKEAGADEVIVSRGLDFASDVRKRTQGAGVDVAVEIVGSAT-FDQTLKSMAPGGRVVVVGNLESGMVQLNPGLVIVKELEI---LGAYATTQAELDEALRLTATGGVRQFVTDAV--PLAEAAKAHFRLENRE-VAGRLVLVPP-

General information: TITO was launched using: RESULT: Template: 5K1S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1768 42156 23.84 139.59 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain C : 0.74 3D Compatibility (PKB) : 23.84 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_5K1S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5K1S-query.scw PDB file : Tito_Scwrl_5K1S.pdb: