Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISKRKHVIIFPILCKKIQGGGSTMIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLL-HVCKTTLLNDINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAVQNK 4EEI Chain:A ((1-433)) ------------------------MIKRYDVAEISKIWADENKYAKMLEVELAILEALEDRMVPKGTAAEIRARAQIRPERVDEIEKVTKHDIIAFCTSIA-----EQFTA------------ETGKFFHFGVTSSDIIDSALSLQIRDSMSYVIKDLEALCDSLLTKAEET-KEIITMGRSH-----GMFAEPMSFGQKFLGAYVEFKRRLKDLKDFQK-DGLTVQFSGAVGNYCILTTEDEKKAADILGLPV---------EEVSTQVIPRDRIAKLISIHGLIAS----AIERLAVEIRHLHR------SDVFEVY---------EGFKKNPISTENLTGMARMLRSHVSIALENCVLWHERDISHSSAERFYLPDNFGIMVYALRRMKNTIDNLVVQRDIIEDRVRS-------TSAYLSSFYLHFLVANTPFMREDCYKIVQQVESFSKKLQKVMHDE---HNIILDIPEMDFEGIKKTYLKEIDHVFDRSVKAR

General information: TITO was launched using: RESULT: Template: 4EEI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1904 -21248 -11.16 -51.45 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -11.16 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.226

(partial model without unconserved sides chains): PDB file : Tito_4EEI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EEI-query.scw PDB file : Tito_Scwrl_4EEI.pdb: