Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQILGGNNMAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIELSKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIY-------------------------GESFEDEF-SRELFNLRGALSMANSG-PNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHVLEGMDTVDEIANVQRGPQ-------DRPVHDVVIETIEISE 4R3F Chain:A ((9-197)) --------------NLEPQPTGAVIIHTTQGDLKVELFAKQTPLTCRNFLQHSLDGYYDGTIFHRLVPGFIIQGGDPTGTGHGGESIYDGGAFSGDLDPWPMDQRKGHNAGPMGVNFKDEFHSRLKFNRRGLLGMANEGAPDTNGSQFFFT---------LGKAEE------------------LNNKNTLFGRVAAG----DTIYNLMKWGEAELIEGTERPQYPVKITNIEI--

General information: TITO was launched using: RESULT: Template: 4R3F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 776 15285 19.70 98.61 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 19.70 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.575

(partial model without unconserved sides chains): PDB file : Tito_4R3F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R3F-query.scw PDB file : Tito_Scwrl_4R3F.pdb: