Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMAIFYQDMTNEEITNLTLAIANSGDVIDLSSL--EGIKVDKHSTGGVGDTTTLILAPLVASVGVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIG-QSGNLAPADKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLAKTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALGSQMVVL-----AGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAI-VEHPE-RILTARYTMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGESLLTIYSNDEE-ITSVIELLYKNIEIGESA-MKPTLIHDIITE 4EAD Chain:A ((2-440)) FLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAMTIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMVAACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQT-SSLAPADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSEALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEY----RNPRLFDVTMALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVEN-YAKYLPTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQVDGQRPLAVIHAKDENNWQEAAKAVKAAIKLADKAPESTPTVYRRISE

General information: TITO was launched using: RESULT: Template: 4EAD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2581 16850 6.53 39.46 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : 6.53 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_4EAD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EAD-query.scw PDB file : Tito_Scwrl_4EAD.pdb: