Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKGITMSE--ISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLVYQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEGFNKQLKKY--TKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF 4WPC Chain:A ((14-310)) ---FSTPEIKKVLNSD--------VAINALLSRLKQSLLTCEEFMKFIRKKYAFEEEHVQELSKQYK-------------HFFNIQNSSLKKMIHEVLGFDGKMAQVKQSYITALQKMYSEISSLLLTMTKLRKSVKENSKRLEKDVSDAIHSAEKAQSRYNSLCQDWDKLRKTKLT-----------------------------------------------------------------------------LRGSKTTKEQEEELLRKIDNADLEYKQKVDHSNSLRNTFITKERPRIVQELKDLILEIDTAMTIQLQ---KYTIWTENLVLNTGVTISPLDSTKSMKSFAGSVSNERDLYSFLNKYNQL----INKNLIPVSYKKHP--

General information: TITO was launched using: RESULT: Template: 4WPC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 625 32296 51.67 115.75 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 51.67 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.216

(partial model without unconserved sides chains): PDB file : Tito_4WPC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WPC-query.scw PDB file : Tito_Scwrl_4WPC.pdb: