Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT-LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITT-NKGIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLD-YFVNDLMKYAGKKVAIAGGGDSAIDWTLMLEPIASEVYLIHRRPEFRAH-EHSVSRLKSSSVNLLTPYLIDGLSGNNGELTDIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDST-RNAITVKSDMSTSIPGVYAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS 3F8D Chain:B ((14-319)) -EKFDVIIVGLGPAAYGAALYSARYMLKTLVIGET--PGGQLTEAGIVD----DYLGLIEIQASDMIKVFNKHIEKYEVPVLLD-IVEKIENR-DEFVVKTKRKGEFKADSVILGIGV-K--RRKLGVPGEQEFAGRGISYCSVADAPLFKNRVVAVIGGGDSALEGAEILSSYSTKVYLIHRRDTFKAQPIYVETVKKKPNVEFVLNSVVKEIKGD-KVVKQVVVENLKTGEIKELNVNGVFIEIGFDPPTDFAKSNGIETDTNGYIKVDEWMRTSVPGVFAAGDCTSAWLGFRQVITAVAQGAVAATSAYRYVTEK-------------------

General information: TITO was launched using: RESULT: Template: 3F8D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1814 -19722 -10.87 -65.74 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -10.87 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.534

(partial model without unconserved sides chains): PDB file : Tito_3F8D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F8D-query.scw PDB file : Tito_Scwrl_3F8D.pdb: