Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSSQEPNQSLFNQASTSIQACIECMFACKHCASACLQEEHVHMMRKCIKHCLTCVETCQLCTSLELRHSELAEQA----MQLCANACQLCAAECSKH--EHEHCQVCAKACLACAQACQAYRA 5NQN Chain:A ((25-127)) ------------------VEMCFDCAQTCAACADACLGEDKVADLRHCIRLNLDCAEICVAAGSIASRAA-TEESILRTMLQTCAEMCRMCEEECRRHAGNHEHCRICADVCKECETACRS---

General information: TITO was launched using: RESULT: Template: 5NQN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 391 -56567 -144.67 -589.24 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -144.67 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.593

(partial model without unconserved sides chains): PDB file : Tito_5NQN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5NQN-query.scw PDB file : Tito_Scwrl_5NQN.pdb: