Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKLTKRQKAIAAAVEANKVYTLEEAVQVLNSLPAA---KFKESLDISVNLGVDPRKSDQVVRGATTLPAGTGKTVRVAVFAQGAQAEAAKEAGADVVGFDDLAESIQGGNL-DFDVVIAAPDAMRVVGKLGTILGPRGLMPNPKVGTVTPDVAGAVKNAKSGQARYRVDKAGIIHAAIGQVGFDAAAIRQNVETLVADLKKLKPATSKGVYIKKITLSSTMGPGLTVDVNNVSS 3QOY Chain:A ((1-233)) MARRGKKYIEASKLVDRNKRYTLEEAVDLLKKMEEVLQRRFDETVELAMRLNVDPRYADQMVRGSVVLPHGLGKPIKVVVFAEGEYAKKAEEAGADYVGGDELINKILKEEWTDFDVAIATPEMMPKVAKLGRILGPRGLMPSPKTGTVTTNVEQAIKDAKRGRVEFKVDKAGNVHMPVGKISFEKEKLIDNLYAAIDAVVRAKPPGAKGQYIKNMAVSLTMSPSVKLDINEV--

General information: TITO was launched using: RESULT: Template: 3QOY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1159 19125 16.50 83.52 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : 16.50 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.594

(partial model without unconserved sides chains): PDB file : Tito_3QOY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QOY-query.scw PDB file : Tito_Scwrl_3QOY.pdb: