Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRHYEIVLLVHPDQSDQVVGMVERYISQIKEADGQIHRLEDWGRRQLAYPINKIHKAHYILMNVECGQSTLDELEELFRYNDAIIRNLIIRREHAITEESLLAKSAEEKRARKAQREEAQQVAQEAE 5UZ4 Chain:F ((1-100)) MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGRRQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEAS---------------------------

General information: TITO was launched using: RESULT: Template: 5UZ4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 419 -67692 -161.55 -676.92 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain F : 0.90 3D Compatibility (PKB) : -161.55 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_5UZ4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UZ4-query.scw PDB file : Tito_Scwrl_5UZ4.pdb: