Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTELQDIDFVELSDEEMHLYSRQILLDGWDIEAQEKLKLANVLIVGAGGIGCSSAELLARAGVGKITLIDADTIEISNLQRQIAFGHEDIGRYKAEVLAKRLQKINPYICVEYFNERLDEHNIDKLVEHQDVVLDGCDNFTTRYLVNAACKKHQVALISASAIGFQAQMFMVEG--DSACYECLFPKEQHANEGLRCAESGVLATTPVMIASLQAHHTLLYLGLNRTPLKQKLLLWDGLNMTQRIVSFDKDINCPFCQAS 1ZKM Chain:A ((3-246)) -----------MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAALYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTALQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMVLTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVG--VMGTLQALEAIKLLSGIETPAGE-LRLFDGKSSQWRSLALRRASGCPVCG--

General information: TITO was launched using: RESULT: Template: 1ZKM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1339 -145358 -108.56 -600.65 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -108.56 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_1ZKM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZKM-query.scw PDB file : Tito_Scwrl_1ZKM.pdb: