Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNFLPAEEQLALIQRGTHEIISEEDLLKKLKENRPLKIKAGFDPTAPDLHLGHTVLINKLKTFQDLGHEVTFLIGDYTAMIGDPTGKSATRPPLSREQVEANAKTYQEQVFKILD----PNKTKVRFNSEWFNQKSAADLIQ-LASQQTVSRMLERDDFTKRYNNHQPIAIHEFLYPLVQGYDSIALE----ADVELGGTDQTFNLLMG-RTLQSRYGQESQVCITVPILEGLDGVNKMSKSLGNYIGVFD----APGAMYQKVLSMPDSLIERYFDLLSFKSLDEIKALLDEIAAGRNPQE--VKRILALELVERFH-DAEAAANAHKSAG-------NRITEGEVPADTPEVTISRGEFGGEIFIATILRVAGLNPNAAAAKDAVARGAVKVDWNVVDASFSVKENGTF------IIQSGKKAIARVTFTD 2JAN Chain:A ((9-420)) ------------LSWRGLIAQSTDLDTLAAEAQRGPMTVYAGFDPTAPSLHAGHLVPLLTLRRFQRAGHRPIVLAGGATGMIGDPR-DVGERSLNEADTVAEWTERIRGQLERFVDFDDSPMGAIVENNLEWTGSLSAIEFLRDIGKHFSVNVMLARDTIRRRLAG-EGISYTEFSYLLLQANDYVELHRRHGCTLQIGGADQWGNIIAGVRLVRQKLGATVH-ALTVPLVTAADG-TKFGKSTGGGSLWLDPQMTSPYAWYQYFVNTADADVIRYLRWFTFLSADELAEL--EQATAQRPQQRAAQRRLASELTVLVHGEAATAAVEHASRALFGRGELARLDEATLAAALRETTVAELKPGSPDGIVDLLVASGLSASKGAARRTIHEGGVSVNNIRVDNEEWVPQSSDFLHGRWLVLRRGKRSIAGV----

General information: TITO was launched using: RESULT: Template: 2JAN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1640 -19435 -11.85 -50.88 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -11.85 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.456

(partial model without unconserved sides chains): PDB file : Tito_2JAN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JAN-query.scw PDB file : Tito_Scwrl_2JAN.pdb: