Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQSVYHIPVKTISGETVDLDQYKGKVLLIVNTASKCGLTP-QYEGLEKLYQAKKDQGLEILGFPANNFKEQEPGSDEEIQQFCSLNYDVHFPLFSKISVAGEDKHPLYQALTTAQPERIGEGPFRERLEGLGIPTNPAPEVLWNFEKFLVNKNGEVIARFAPNLTADDEQIVKAVEAELAK 5H5Q Chain:A ((10-154)) -ARSMHEFSAKDIDGHMVNLDKYRGFVSIVTNVASQ-GKTEVNYTQLVDLHARYAERGLRILAFPSNQFGKQEPGSNEEIKEFAA-GYNVKFDMFSKIEVNGDDAHPLWKWMKI-QP------------KGKGILGNA---IKWNFTKFLIDKNGVVVKRYGP-------------------

General information: TITO was launched using: RESULT: Template: 5H5Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 696 -414 -0.59 -2.90 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -0.59 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.616

(partial model without unconserved sides chains): PDB file : Tito_5H5Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5H5Q-query.scw PDB file : Tito_Scwrl_5H5Q.pdb: