Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITTSVTEAAECLQQGQVLAYPTEAVWGLGCDPFNEQAFQKILELKQRPIEKGVILLAGHISQVEHLLTSLPQTTQQEIIDCWTNHQPSERATTWLLPADQHIPSWIKGEHPLVAVRVTTHPLCVALCNAFHGFIVSTSANPSGQEPAHSLQDACQYFGSQLNYLNGDLGQSQQPSRIINALTGEVIRP 2EQA Chain:A ((20-166)) --------AADVIRNGGTVAFPTETVYGLGANAFDGNACLKIFQAKNRPVDNPLIVHIADFNQLFEVAKDIPDKVLEIAQIVWPG------PLTFVLKKTERVPKEVTAGLDTVAVRMPAHPIALQLIRESGVPIAAPSAN------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2EQA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 668 -105579 -158.05 -831.33 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -158.05 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.530

(partial model without unconserved sides chains): PDB file : Tito_2EQA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EQA-query.scw PDB file : Tito_Scwrl_2EQA.pdb: