Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDILENPLELCGFAFIEFVSKENELDPIFETIGFSKVAKHKSKKAYLWRQGNINIILNYQPESY---ASFFFNEHGPSACAMGFKTRDAAKAFKKAVELGAEPMYS--KVGPMELNIPAIKGIGGMPIFLVDCDIYEND----FVFFDDAQRNPEG-AGLKEIDHLTHNVYKGRMEYWANFYEKIFNFQEIRYFDIKGEYTGLTSKALTAPDGMIRIPLNE-DSDKGNGQIAEFLADFNGEGIQHIAFICDDLISTWDKLKGLGLKFMTPPPNTYYEMLEERLPEHGEPTEELKQRCILLDGSTKDGQKKLLLQIFSENM--IGPVFFEFIQRKDDDGFGEGNFKALFESIERDQIRRGVLEAK 2R5V Chain:A ((13-343)) ---VEN-LEVAAFSWVDKYA--------FAVAGTSRSADHRS---IALRQGQVTLVLT-EPTSDRHPAAAYLQTHGDGVADIAMATSDVAAAYEAAVRAGAEAVRAPGQHS-AAVTTATIGGFGDVVHTLIQRDGTSAELPPGFTGSMDVTNHGKGDVDLLGIDHFAICLNAGDLGPTVEYYERALGFRQIFDEHIVVGAQAMNSTVVQSASGAVTLTLIEPDRNADPGQIDEFLKDHQGAGVQHIAFNSNDAVRAVKALSERGVEFL-KTPGAYYDLLGERITLQTHSLDDLRATNVLAD-EDHGGQ---LFQIFTASTHPRHTIFFEVIERQGAGTFGSSNIKALYEAVE------------

General information: TITO was launched using: RESULT: Template: 2R5V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1659 -17514 -10.56 -55.25 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -10.56 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.333

(partial model without unconserved sides chains): PDB file : Tito_2R5V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2R5V-query.scw PDB file : Tito_Scwrl_2R5V.pdb: