Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHLNSSIDKKMTTLNILFKTTSLCLAMALLVGCNDNDDNDSSTPAPRSNQTVNILAFNDFHGNLEPPKRYVEAANPNDASQTVRIPVGGVSYFADAIKK-LKAQNPNNVVVSAGDLISASPLTSSLFLDEPTIEVMNDIQIDFNAVGNHEFDRGTDELRRLQNGGCQQYTSTAPCQINKNFSGAKFNFLAANVAMKNDTSKTIFPAYKVKTYGGIPVAFIGLTLKATPSIVSAAGIKDVEFRDEADTVNALIPELQK-QGIEAIVVVVHEGAAPSTKLNQKTCDGLSGPILGILDRLNPA--VDIVVSGHTHQSYIC---------DYATKNPAKP-----FLLTSAGQYGTLITDIKVGLDGKTGDIIKKDAKQIPVQSEAYTSGTTTVSLTDLYQ-KFSKTPSIEAILDKYRQ--------AVTTISGRVVGTSTAVVSRTQVESGESPLGDMIADAQQAAALQASNQGSDFTLMNPGGVRADLLINSSNQITFGDIFAVQPFGNSIVTLSLTGKQIRDVLEQQWSGANANSPRILQPSKELSYQYAANTSVSP--RASNIMVAGSPLVDTKVYRVTVNSFLADGGDNFTVLKEGQNRVGGG-QDIDALESYVAQNSKDSPLIIPATTRIKVIK 4WWL Chain:A ((37-511)) --------------------------------------------------------------------------------------------LVDGIRKEVAAEGGSVLLLSGGDINTGVP-ESDLQDAEPDFRGMNLVGYDAMAIGNHEFDNPLTVLRQQE-------------------KWAKFPLLSANIYQKS-TGERLFKPWALFKRQDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHMGHYDNGE------HGSNAPGDVEMARALPAGSLAMIVGGHS-QDPVCMAAENKKQVDYVPGTPCKPDQQNGIWIVQAHEWGKYVG--RADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVLYTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEG------DRDKVRFVQTNMGRLI------LAAQMDRTGADFAVMSGGGIRDSI---EAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYL---------TAVAQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPRLDNKPGYVNTGFCDAEVLKAYI---QKSSPL------------

General information: TITO was launched using: RESULT: Template: 4WWL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2340 60931 26.04 136.92 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 26.04 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_4WWL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WWL-query.scw PDB file : Tito_Scwrl_4WWL.pdb: