Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNALTQELVELL-TLEKLEENIYRGISRNLVG--KRVFGGQVLGQALRAASYT-TDRPAHSLHAYFLYGGDINAPIIYEVDRLRDGKSFVSRQVRAIQHGRVIFSAMVSFA-NPEEGLNYQHPEPDYPAPEALKSESELKEGILNFVPENVRASFMRERHVEIRPIDPVNPFQPQPEAPFNAHYIRTHDRIPKQLEDISLHQAIVAFYSDFTLMTTALRPHGLSYISPSLQCASIDHAIYFHRPLRADEWMLYDMEATVSAASRGLNFGRMW-QNGQLVCSTVQEGLMRLREIETQ 4R9Z Chain:A ((4-267)) ------SLGDVLATLELERVGQWRFVGQQLPAPANHILGGHISAQALLAASRTAAGREPHSVHTYFLRPGDSRQPVDFEVVDLQEGRTFSARRVTARQDDKILMEAMSSFKV----QVVYQPIMPEAPSPESL----------------------ASLRWFERRTIE--TETVPPARVPM---WWRPDGRVP---DDPVLTASLVAYMSAVTLTEPAFAARGGVGAS-----AQRDHSVWFHGRAVLSDWLFYDRSSPSSAGSLALASGTMFNRTGELVCTVKQE-----------

General information: TITO was launched using: RESULT: Template: 4R9Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1290 -7194 -5.58 -30.74 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -5.58 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.321

(partial model without unconserved sides chains): PDB file : Tito_4R9Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R9Z-query.scw PDB file : Tito_Scwrl_4R9Z.pdb: